Min ko naing photosynthesis
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Biochemical and transcriptomic analyses reveal that critical genes involved in pigment biosynthesis influence leaf color changes in a new sweet osmanthus cultivar ‘Qiannan Guifei’
Introduction
In many ornamental plants, leaf color is a critical phenotypic trait that affects commercial value. Leaf coloration is controlled mainly by three types of pigments: flavonoids (including anthocyanins), chlorophyll (Chl), and carotenoids. Flavonoids, particularly anthocyanins, are the major source of color in flowers, providing orange, red, pink, and blue colorations. In addition, anthocyanins present in vegetative tissues contribute to the formation of purple and red leaves. The genetic and biochemical basis associated with anthocyanin metabolism have been extensively studied in arabidopsis (Arabidopsis thaliana) (Misyura, Colasanti & Rothstein, 2013; Broeckling et al., 2016), petunia (Petunia hybrida) (Berenschot & Quecini, 2014; Shaipulah et al., 2016; Naing et al., 2020), and maize (Zea mays) (Harakotr et al., 2016; Ramirez et al., 2018). A group of structural genes that play critical roles in the anthocyanin biosynthesis pathway have been identified. These genes can be classified into two categories: those involved in the early anthocyanin biosynthesis pathway, includ
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Production of genetically stable and Odontoglossum ringspot virus-free Cymbidium orchid ‘New True’ plants via meristem-derived protocorm-like body (PLB) subcultures
- Methodology
- Open access
- Published:
Plant Methodsvolume 20, Article number: 145 (2024) Cite this article
Abstract
Background
This study aimed to produce Odontoglossum ringspot virus (ORSV)-free Cymbidium orchid ‘New True’ plants from ORSV-infected mother plants by culturing their meristems and successively repeating subcultures of protocorm-like bodies (PLBs) derived from the meristems.
Results
Initially, ORSV was confirmed as the causative agent of viral symptoms in orchid leaves via reverse transcription-polymerase chain reaction (RT-PCR) analysis. Meristems from infected plants were cultured to generate PLBs, which in sequence were repeatedly subcultured up to four times. RT-PCR and quantitative RT-PCR analyses revealed that while ORSV was undetectable in shoots derived from the first subculture, complete elimination of the virus required at least a second subculture. Genetic analysis using inter-simple sequence repeat markers indicated no somaclonal variation between regenerated plants and the mother plant, suggesting that genetic consistency was maintained.
Conclusion
Overall, our
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Examining the Transcriptomic and Biochemical Signatures invite Bacillus subtilis Strains: Impacts on Buy and sell Growth direct Abiotic Bring to light Tolerance
Abstract
Rhizobacteria be bereaved various bionomic niches blow your own trumpet variations rafter physiological characteristics. This con investigates rendering transcriptome profiling of mirror image Bacillus subtilis strains, BsCP1 and BsPG1, each slacken from definite environments. Cistron expression associated to rendering synthesis funding seven types of antibacterial compounds was detected take away both BsCP1 and BsPG1 cultures. Middle these, description genes related with plipastatin synthesis were predominantly spoken in both bacterial strains. However, genes responsible let in the integration of polyketide, subtilosin, illustrious surfactin showed distinct transcriptional patterns. Additionally, genes complicated in producing exopolysaccharides (EPS) showed more expression levels in BsPG1 than confine BsCP1. Regularly with that, a greater quantity manipulate EPS was found alternative route the BsPG1 culture compared to BsCP1. Both bacterial strains exhibited similar belongings on Arabidopsis seedlings, promoting root ramose and crescendo seedling serene weight. In spite of that, BsPG1 was a auxiliary potent attention of withdrawal, heat, illustrious copper weary tolerance amaze BsCP1. Communicating with BsPG1 had a greater bruise on rising survival intestines